Instructors: Garrett Odell (GMO; odellgm@u.washington.edu), Ed Munro (EMM; munroem@u.washington.edu), George von Dassow (GvD; dassow@u.washington.edu), Kristin Sherrard (KS; kmsherra@midway.uchicago.edu)
| Day | Time | Event | Location | Instructor |
|---|---|---|---|---|
| 9/29 | 9 am | Orientation and purpose of the course | Commons | All |
| 9:30 am | Lecture: Introduction to Embryogenesis | Commons | GvD | |
| 10:15 am | Lecture: Introduction to Cell Mechanics | Commons | EMM | |
| 11 am | Lecture: Introduction to Dynamical Systems | Commons | GMO | |
| 12:15 pm | Lunch | |||
| 1 pm | Tour | KS | ||
| 2:30 pm | Math Lab - Using Mathematica | Lab 4 | ||
| 4:30 pm | Tea & Mixer - say who you are, where you come from, what you're interested in, etc. | Lab 4 | ||
| 9/30 | 9 am | Lecture - Embryology of Drosophila | Commons | GvD |
| 10:30 am | Lab - Embryology of Drosophila; learn to collect eggs, dechorionate, observe embryos with the microscope; learn basic microscope skills | Lab 4 | GvD & VEF | |
| 1 pm | Drosophila lab continues | Lab 4 | ||
| 4 pm | Tea | |||
| 10/1 | 9 am | Paper discussion - Driever bicoid gradient paper | Commons | GvD |
| 10:30 am | Math Lecture | Commons | GMO | |
| 1 pm | Math Lab | Lab 4 | ||
| 4 pm | Tea | |||
| 10/2 | 9 am | Lecture - Embryology of C. elegans | Commons | EMM |
| 10:30 am | Lab - Embryology of C. elegans; learn to manipulate worms, make tools, collect and observe embryos; learn to use DIC scope | Lab 4 | EMM | |
| 1 pm | C. elegans lab continues | Lab 4 | ||
| 4 pm | Tea | |||
| 10/3 | 9 am | Paper discussion - Goldstein gut induction paper | Commons | EMM |
| 10:30 am | Math Lecture | Commons | GMO | |
| 1 pm | Math Lab | Lab 4 | ||
| 4 pm | Tea | |||
| 10/4 (Sat) | Apple Raid | SV Melibe / Shaw Island | ||
| Week 2 | ||||
| 10/6 | 9 am | Paper discussion - Vincent engrailed paper | Commons | GvD |
| 10:30 am | Math Lecture | Commons | GMO | |
| 1 pm | Math Lab | Lab 4 | ||
| 4 pm | Tea | |||
| 10/7 | 9 am | Lecture - Embryology of ascidians | Commons | GvD |
| 10:30 am | Lab - Embryology of ascidians; harvest Corella broods, observe embryos; obtain gametes from Boltenia, observe cytoplasmic segregation and cleavage cycles; learn to make time-lapse movies | Lab 4 | EMM & GvD | |
| 1 pm | Ascidian lab continues | Lab 4 | ||
| 4 pm | Tea | |||
| 10/8 | 9 am | Paper discussion - Nishida cytoplasm transplantation paper | Commons | EMM |
| 10:30 am | Math Lecture | Commons | GMO | |
| 1 pm | Math Lab | Lab 4 | ||
| 4 pm | Tea | |||
| 4:30 pm | CCD Lab Meeting | Commons | ||
| 10/9 | 9 am | Lecture - Establishment of AP polarity in C. elegans | Commons | EMM |
| 10:30 am | Lab - GFP worms; introduction to the confocal imaging; learn to mount embryos; time-lapse confocal on Radiance and CARV using myosin-GFP, tubulin-GFP, etc. | Lab 4 | EMM + (GvD & GMO & PB) | |
| 1 pm | GFP worm lab continues | Lab 4 | ||
| 4 pm | Tea | |||
| 10/10 | 9 am | Paper discussion - Ed's new worm paper | Commons | EMM |
| 10:30 am | Math Lecture | Commons | GMO | |
| 1 pm | Math Lab | Lab 4 | ||
| 10/11 (Sat) | 9 am | Presentations - Show & Tell; movies, photos, observations from first two weeks; instructors will provide refreshments | Commons | Students |
| 4 pm | Tea | |||
| Week 3 | ||||
| 10/13 | 9 am | Paper discussion - Heemskerk engrailed paper | Commons | Students |
| 10:30 am | Math Lecture | Commons | GMO | |
| 1 pm | Math Lab | Lab 4 | ||
| 4 pm | Tea | |||
| 10/14 | 9 am | Lecture - Pattern formation in Drosophila | Commons | GvD |
| 10:30 am | Lab - Drosophila embryos, fixation and antibody staining for dorsal, eve, slp, en, et al. | Lab 4 | GvD & VEF | |
| 1 pm | Lab continues | |||
| 4 pm | Tea | |||
| 10/15 | 9 am | Paper discussion - Sternberg vulval induction | Commons | Students |
| 10:30 am | Math Lecture | Commons | GMO | |
| 1 pm | Math Lab | Lab 4 | ||
| 4 pm | Tea | |||
| 4:30 pm | CCD Lab Meeting | Commons | ||
| Evening | Mount stained fly embryos; examine preps in evenings or otherwise as time allows | Lab 4 | ||
| 10/16 | 9 am | Lecture - Cell adhesion in morphogenesis | Commons | EMM |
| 10:30 am | Lab - ascidian embryos; dechorionation, dissociation, DiI or FM 1-43 labeling, beads | Lab 4 | EMM & GvD | |
| 1 pm | Lab continues | Lab 4 | ||
| 4 pm | Tea | |||
| 10/17 | 9 am | Paper discussion - Townes & Holtfreter tissue affinity paper | Commons | Students |
| 10:30 am | Math Lecture | Commons | GMO | |
| 1 pm | Math Lab | Lab 4 | ||
| 4 pm | Tea | |||
| 10/18 (Sat) | 9 am | Presentations - Show & Tell; movies, photos, observations from first two weeks; instructors will provide refreshments | Commons | Students |
| Week 4 | ||||
| 10/20 | 9 am | Paper discussion - Pritchard MBT paper | Commons | Students |
| 10:30 am | Math Lecture - Actin networks | Commons | EMM | |
| 1 pm | Math Lab - Network mechanics | Lab 4 | EMM | |
| 4 pm | Tea | |||
| 10/21 | 9 am | Guest Lecture - Jonathon Alberts on Listeria motility | Commons | |
| 10:30 am | Lab - Fixing and staining worm embryos | Lab 4 | EMM | |
| 1 pm | Lab - Worm fixation continues | Lab 4 | ||
| 4 pm | Tea | |||
| 10/22 | 9 am | Paper discussion - George's nuclear migration paper | Commons | Students |
| 10:30 am | Math Lecture - Gene network models | Commons | GvD | |
| 1 pm | Math Lab - Ingeneue | Lab 4 | GvD | |
| 4 pm | Tea | |||
| 4:30 pm | CCD Lab Meeting | Commons | ||
| 10/23 | 9 am | Lecture - More gene networks | Commons | GvD |
| 10:30 am | Lab - Microinjections; inject worms with DiI, RNAi, Dextrans; inject fly embryos with various things | Lab 4 | EMM & GvD | |
| 1 pm | Lab continues | Lab 4 | ||
| 4 pm | Tea | |||
| 10/24 | 9 am | Discuss possible course projects | Commons | |
| 1 pm | Lab - Confocal training day; everyone spend time on each of the CARV, Radiance, and MRC600; continue through evening and weekend as time allows | Lab 4, 8, 9 | GvD, EMM, GMO, PB | |
| 4 pm | Tea | |||
| 10/25 | 9 am | Presentations | Commons | Students |
| Week 5 | ||||
| 10/27 | 10:30 am | Lecture - Optics I: Basics of microscopy | Commons | GMO |
| 1 pm | Computer Lab - Optics and ray tracing | Lab 4 | ||
| 4 pm | Tea | |||
| 10/28 | 9 am | Lecture - Mechanics of cell crawling and tissue morphogenesis | Commons | EMM |
| 4 pm | Tea | |||
| 10/29 | 9 am | Paper discussion - ? | Commons | Students |
| 10:30 am | Lecture - Optics II: Fluorescent probes and confocal microscopy | Commons | GMO | |
| 1 pm | Computer Lab - More optics | Lab 4 | ||
| 4 pm | Tea | |||
| 4:30 pm | CCD Lab Meeting | Commons | ||
| 10/30 | 9 am | Lecture - Cytokinesis | Commons | GvD |
| 4 pm | Tea | |||
| 10/31 | 10:30 am | Lecture - Image processing | Commons | GMO |
| 1 pm | Computer Lab - Image processing methods | Lab 4 | ||
| 4 pm | Tea | |||
| 11/1 | 9 am | Presentations | Commons | Students |
| Week 6 | ||||
| 11/3 | 9 am | Lecture - Signal transduction | Commons | GvD |
| 4 pm | Tea | |||
| 11/4 | ||||
| 4 pm | Tea | |||
| 11/5 | 9 am | Paper discussion - NF-kB signaling | Commons | Students |
| 10:30 am | Math Lecture | Commons | GMO | |
| 1 pm | Math Lab | |||
| 4 pm | Tea | |||
| 4:30 pm | CCD Lab Meeting | Commons | ||
| 11/6 | ||||
| 4 pm | Tea | |||
| 11/7 | 9 am | Lecture - PAR proteins as a conserved cell polarity module | Commons | EMM |
| 4 pm | Tea | |||
| 11/8 | 9 am | Presentations | Commons | Students |
| Week 7 | ||||
| 11/10 | 9 am | Lecture - ? | Commons | ? |
| 4 pm | Tea | |||
| 11/11 | Holiday - Veterans' Day | |||
| 11/12 | 9 am | Paper discussion - PAR proteins | Commons | Students |
| 10:30 am | Math Lecture - Numerical solution of differential equations | Commons | GvD | |
| 1 pm | Math Lab - Solving ODEs; solving PDEs | Lab 4 | ||
| 4 pm | Tea | |||
| 4:30 pm | CCD Lab Meeting | Commons | ||
| 11/13 | 9 am | Guest Lecture - Lesilee Rose | Commons | |
| 4 pm | Tea | |||
| 11/14 | ||||
| 4 pm | Tea | |||
| 11/15 | 9 am | Presentations | Commons | Students |
| Week 8 | ||||
| 11/17 | 9 am | Lecture - Intracellular traffic | Commons | GvD |
| 4 pm | Tea | |||
| 11/18 | ||||
| 4 pm | Tea | |||
| 11/19 | 9 am | Paper discussion - | Commons | Students |
| 10:30 am | Math Lecture - Case study | Commons | ||
| 4 pm | Tea | |||
| 4:30 pm | CCD Lab Meeting | Commons | ||
| 11/20 | ||||
| 4 pm | Tea | |||
| 11/21 | 9 am | Guest Lecture - Lance Davidson | Commons | |
| 4 pm | Tea | |||
| 11/22 | 9 am | Presentations | Commons | Students |
| Week 9 | ||||
| 11/24 | 9 am | Guest Lecture - George Oster | ||
| 4 pm | Tea | |||
| 11/25 | ||||
| 4 pm | Tea | |||
| 11/26 | 9 am | Paper discussion | Commons | Students |
| 10:30 am | Math Lecture - Case study | Commons | ||
| 4 pm | Tea | |||
| 4:30 pm | CCD Lab Meeting | Commons | ||
| 11/27 | Holiday - Thanksgiving | |||
| 11/28 | Holiday again | |||
| 11/29 | Holiday some more | |||
| Week 10 | ||||
| 12/1 | ||||
| 4 pm | Had enough tea yet? | |||
| 12/2 | ||||
| 4 pm | Tea | |||
| 12/3 | 9 am | Paper discussion | Commons | Students |
| 10:30 am | Math Lecture - Case study | Commons | ||
| 4 pm | Tea | |||
| 4:30 pm | CCD Lab Meeting | Commons | ||
| 12/4 | ||||
| 4 pm | Tea | |||
| 12/5 | ||||
| 4 pm | Tea | |||
| 12/6 | ||||
| Week 11 | ||||
| 12/8 | ||||
| 12/9 | Last day to hand in notebooks/papers | |||
| 12/10 | Final Presentations | |||
| 12/11 | LAB CLEAN-UP: nobody goes home until Lab 4 is clean and all equipment put away | |||
